UW School of Medicine Microbiology Professor Evgeni Sokurenko helps with the efforts to identify and track variants of the COVID-19 virus. Alongside Seattle-based ID Genomics, which Sokurenko founded, the professor’s laboratory is leading the creation and future implementation of a new method to “fingerprint” all currently known variants.
The method identifies the presence of dozens of mutations at once and requires only three ingredients: a dipstick, a common thermocycler instrument and a processed test sample.
Upon coming into contact with the sample, the dipstick records which variant is present by exposing a series of lines, or bands. Every known variant of interest and concern has a correlating band pattern, which the test administrator can match to the sample.
“It's a rapid test. We can identify the variant within two hours of the sample being in hand,” says Sokurenko.
Currently the definitive method of tracking variants of the virus, including the delta variant, is done through genomic sequencing. The process is thorough but can take up to two weeks to collect a sufficiently large batch of samples and yield results. Sokurenko’s method provides high-resolution results much more quickly, with a single or a few samples in hand.
A grant awarded through the National Institutes of Health helped with the development of the test, which will also include a barcode component that can be scanned through a smartphone app. The app connects the user to a database of all variant fingerprints. Preprogrammed and battery-capable thermocyclers, developed by Seattle’s IEH Laboratories, will also be made available for distribution with test materials.
Sokurenko says the test is already available as a prototype in the form of a same-day to next-day service in his lab on a collaborative basis, while a wider distribution of the full kit for clinical epidemiologists and researchers could come by this winter. He says the method is also designed with a key ability: potentially tracking new variants as they emerge.